Part:BBa_K1217000:Design

pT7-ppk1(T.forsythia)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 364
  Illegal BglII site found at 1826
  Illegal XhoI site found at 108
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1284
  Illegal NgoMIV site found at 1596
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 384
  

Design Notes

We choose to amplify ppk1 gene from Tannerella forsythia (ATCC 43037) since the sequence does not contain any of the illegal restriction sites.

Source

The coding gene ppk1(E.C. 2.7.4.1) is amplified from Tannerella forsythia (ATCC 43037) genomic DNA.